A patient presents with signs of anemia, and the provider has ordered a complete blood count (CBC). After performing venipuncture, you must prepare the specimen for transport to the laboratory. Which of the following steps is appropriate for ensuring the validity of the blood sample?
Shake the blood sample vigorously to ensure proper mixing of the blood with additives.
Allow the blood to sit for 10 minutes before capping the tube to prevent hemolysis.
Add a few drops of heparin to the blood sample to prevent clotting before transportation.
Gently invert the tube containing EDTA 6-10 times to mix the sample thoroughly.
The blood should be gently inverted 6-10 times to mix the blood with the anticoagulant and prevent clotting without causing hemolysis. Shaking the blood vigorously may cause hemolysis and thus affect the test results. Using other additives may lead to sample contamination or incorrect test results because they may not be suitable for hematology tests like CBC, which often require EDTA as an anticoagulant.
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